@article {Zhang:2020:0143-2044:100,
title = "Hypothermic Storage of Human Umbilical Cord Mesenchymal Stem Cells and Their Hydrogel Constructs",
journal = "Cryoletters",
parent_itemid = "infobike://cryo/cryo",
publishercode ="cryo",
year = "2020",
volume = "41",
number = "2",
publication date ="2020-03-01T00:00:00",
pages = "100-105",
itemtype = "ARTICLE",
issn = "0143-2044",
eissn = "1742-0644",
url = "https://www.ingentaconnect.com/content/cryo/cryo/2020/00000041/00000002/art00007",
keyword = "ALGINATE, PVA, HUMAN UMBILICAL CORD MESENCHYMAL STEM CELLS, ENCAPSULATION, HYPOTHERMIC STORAGE",
author = "Zhang, Xiaozhang and Zhao, Gang",
abstract = "
BACKGROUND: Human umbilical cord mesenchymal stem cells (hUMSCs) and hUMSCs-hydrogel constructs have great potential in clinical application. However, research about hypothermic storage of hUMSCs and hUMSCs-hydrogel constructs has not been fully investigated. OBJECTIVE:
To evaluate the cell survival without and with encapsulation (alginate, alginate with PVA) at 4 \textdegreeC and 25 \textdegreeC, respectively. MATERIALS AND METHODS: Acridine orange/ethidium bromide (AO/EB) staining and fluorescence staining were used to evaluate cell viability during hypothermic
storage. RESULTS: Cells with encapsulation show significantly higher viability than cells without encapsulation at 4 \textdegreeC, while there was no obvious difference between viability of cells without and with encapsulation at 25 \textdegreeC. In addition, cells encapsulated with alginate and
PVA show better viability than cells encapsulated with alginate alone. Moreover, cell viability at 25 \textdegreeC was significantly higher than cell viability at 4 \textdegreeC. CONCLUSION: hUMSCs aggregates and hUMSCs-hydrogel constructs can survive for 7 days by hypothermic storage at 25 \textdegreeC,
which facilitates the cell delivery and application of hUMSCs and hUMSCs-hydrogel constructs in clinical medicine.",
}