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Hypothermic Storage of Human Umbilical Cord Mesenchymal Stem Cells and Their Hydrogel Constructs

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BACKGROUND: Human umbilical cord mesenchymal stem cells (hUMSCs) and hUMSCs-hydrogel constructs have great potential in clinical application. However, research about hypothermic storage of hUMSCs and hUMSCs-hydrogel constructs has not been fully investigated. OBJECTIVE: To evaluate the cell survival without and with encapsulation (alginate, alginate with PVA) at 4 °C and 25 °C, respectively. MATERIALS AND METHODS: Acridine orange/ethidium bromide (AO/EB) staining and fluorescence staining were used to evaluate cell viability during hypothermic storage. RESULTS: Cells with encapsulation show significantly higher viability than cells without encapsulation at 4 °C, while there was no obvious difference between viability of cells without and with encapsulation at 25 °C. In addition, cells encapsulated with alginate and PVA show better viability than cells encapsulated with alginate alone. Moreover, cell viability at 25 °C was significantly higher than cell viability at 4 °C. CONCLUSION: hUMSCs aggregates and hUMSCs-hydrogel constructs can survive for 7 days by hypothermic storage at 25 °C, which facilitates the cell delivery and application of hUMSCs and hUMSCs-hydrogel constructs in clinical medicine.
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Keywords: ALGINATE; ENCAPSULATION; HUMAN UMBILICAL CORD MESENCHYMAL STEM CELLS; HYPOTHERMIC STORAGE; PVA

Document Type: Research Article

Publication date: March 1, 2020

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  • CryoLetters is a bimonthly international journal for low temperature sciences, including cryobiology, cryopreservation or vitrification of cells and tissues, chemical and physical aspects of freezing and drying, and studies involving ecology of cold environments, and cold adaptation

    The journal publishes original research reports, authoritative reviews, technical developments and commissioned book reviews of studies of the effects produced by low temperatures on a wide variety of scientific and technical processes, or those involving low temperature techniques in the investigation of physical, chemical, biological and ecological problems.

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