Provider: Ingenta Connect
Database: Ingenta Connect
Content: application/x-research-info-systems
TY - ABST
AU - Santos, Kamilla M.
AU - Maciel, Fernanda P.S.
AU - Souza, Cristina M.
AU - Cassali, Geovanne D.
AU - Thomé, Ralph G.
AU - Santos, Hélio B.
AU - Ribeiro, Rosy. I. M. A
TI - Evaluation of Different Methods of Cryopreservation of Ehrlich Tumor Cells
JO - Cryoletters
PY - 2015-03-01T00:00:00///
VL - 36
IS - 2
SP - 68
EP - 73
KW - TRANSPLANTABILITY
KW - TREHALOSE
KW - HEN EGG YOLK
KW - ASCITIC TUMOR
KW - CRYOPROTECTIVE AGENT
KW - GLYCEROL
N2 -
BACKGROUND: The Ehrlich Ascitic Carcinoma (EAC) is an experimental transplantable neoplasm that develops in several species of mice. The maintenance of the tumor occurs in vivo. Thus, freezing the cells would reduce the number of passages between animals, ensuring genetic
stability and storage for longs period of experimentation. OBJECTIVE: Search by EAC cryoprotectants. MATERIALS AND METHODS: The combinations of nutrient medium (Tris, hen egg yolk, and DEMEM) and cryoprotective agent (Glycerol, Trehalose and DMSO) on freezing EAC cells and the
transplantability after defrosting were evaluated. The cooling was conducted at 2°C/min. until -180°C and the thawing by immersion in water at 37°C. The transplantability was evaluated from cell inoculation in mice for 14 days. RESULTS: The best results were the associations
IA (Cryoprotective agent Glycerol 6% and medium containing 3.0% Tris w / v, 1.8% Citric acid w / v, 1.3% D-fructose w / v and 20% hen egg yolk v / v) and IIB (Cryoprotective agent Trehalose 100mM and medium containing 50% coconut water v / v, 25% sodium citrate 5% v / v and 20% hen egg yolk
v / v) with 85.2% and 55.1% viable cells, respectively. CONCLUSION: These transplantable cells were efficient for tumor development, therefore demonstrating that this method of cryopreservation is simple and affordable.
UR - https://www.ingentaconnect.com/content/cryo/cryo/2015/00000036/00000002/art00001
ER -