@article {Jitsopakul:2008:0143-2044:253,
title = "Cryopreservation of Vanda coerulea Protocorms by Encapsulation-Dehydration",
journal = "Cryoletters",
parent_itemid = "infobike://cryo/cryo",
publishercode ="cryo",
year = "2008",
volume = "29",
number = "3",
publication date ="2008-05-01T00:00:00",
pages = "253-260",
itemtype = "ARTICLE",
issn = "0143-2044",
eissn = "1742-0644",
url = "https://www.ingentaconnect.com/content/cryo/cryo/2008/00000029/00000003/art00008",
keyword = "ENCAPSULATION-DEHYDRATION, VANDA COERULEA, MORPHOLOGICAL VARIATION, PLOIDY LEVEL, CRYOPRESERVATION, PROTOCORMS",
author = "Jitsopakul, Nipawan and Thammasiri, Kanchit and Ishikawa, Keiko",
abstract = "Protocorms of Vanda coerulea were successfully cryopreserved by encapsulation-dehydration in combination with a loading solution. Protocorms were selected 70 days after sowing seeds harvested from 7-month-old fruits. After encapsulation in an alginate matrix composed of 2% Na-alginate, 2 M glycerol plus 0.4 M sucrose (loading solution), the protocorms were precultured in modified Vacin and Went (1949) (VW) liquid medium supplemented with 0.7 M sucrose on a shaker (110 rpm) at 25 \textpm 3\textdegree C for 20 h. Encapsulated protocorms were then dehydrated in a sterile air-flow in a laminar air-flow cabinet at 25 \textpm 3\textdegreeC for 0-10 h and then directly plunged into liquid nitrogen for 1 d. After thawing at 40\textdegreeC for 2 min, cryopreserved beads were cultured on modified VW agar medium for regrowth. The highest regrowth of 40% was observed with cryopreserved beads with 35% water content after 8 h dehydration. No morphological variation was detected between non-cryopreserved and cryopreserved plantlets, and ploidy level was unchanged as a result of cryopreservation.",
}