The effect of cryopreservation on human spermatozoa has been investigated during the past few decades. The majority of current cryopreservation protocols are carried-out using low cooling rates. However, theoretical calculations have shown that for human spermatozoa an optimal cooling rate is about 7x103 °C/min. In our work we have studied the effect of cryopreservation with high cooling rates, variation of osmolarity of the cryoprotectant medium and the glycerol content. The results of experiments have demonstrated that within the range of high cooling rates, after thawing the dependency of sperm survival on the cooling rate has a maximum recovery at 2.5-3.3 x103 °C/min in moderately hyperosmolar medium, containing 4-5% glycerol. When decreasing the cooling rate down to 1.75-2.5 x103 °C/min there was a statistically significant reduction in sperm motility. Using the cooling rate of 8-11 x103 °C/min only a small percentage of spermatozoa retained their motility.
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Document Type: Research Article
Institute for Problems of Cryobiology & Cryomedicine of the National Academy of Sciences of Ukraine, 23 Pereyaslavskaya str., Kharkov 61015, Ukraine
March 1, 2003
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CryoLetters is a bimonthly international journal for low temperature sciences, including cryobiology, cryopreservation or vitrification of cells and tissues, chemical and physical aspects of freezing and drying, and studies involving ecology of cold environments, and cold adaptation
The journal publishes original research reports, authoritative reviews, technical developments and commissioned book reviews of studies of the effects produced by low temperatures on a wide variety of scientific and technical processes, or those involving low temperature techniques in the investigation of physical, chemical, biological and ecological problems.