@article {Gagliardi:2002:0143-2044:61, title = "Cryopreservation of cultivated and wild arachis species embryonic axes using desiccation and vitrification methods", journal = "Cryoletters", parent_itemid = "infobike://cryo/cryo", publishercode ="cryo", year = "2002", volume = "23", number = "1", publication date ="2002-01-01T00:00:00", pages = "61-68", itemtype = "ARTICLE", issn = "0143-2044", eissn = "1742-0644", url = "https://www.ingentaconnect.com/content/cryo/cryo/2002/00000023/00000001/art00009", keyword = "GERMPLASM PRESERVATION, DESICCATION, GROUNDNUT, EXTRANERVOSAE, PEANUT, CRYOPRESERVATION", author = "Gagliardi, R.F. and Pacheco, G.P. and Valls, J.F.M. and Mansur, E.", abstract = "The effects of two methods of cryopreservation involving chemical vitrification and air desiccation) were studied on isolated embryonic axes of A. hypogaea. Vitrification with PVS2 and desiccation in a laminar flow cabinet resulted in high levels (70-90%) of whole plant recovery after cryopreservation. A desiccation protocol based on 1h exposure of explants to the air flow was successfully applied to six wild species of section Extranervosae, resulting in recovery levels of 70-90% after liquid nitrogen treatment.", }