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Molecular analyses were carried out on four preflight and six postflight International Space Station (ISS)-associated potable water samples at various stages of purification, storage, and transport, to ascertain their associated microbial diversities and overall microbial burdens. Following DNA extraction, PCR amplification, and molecular cloning procedures, rDNA sequences closely related to pathogenic species of Acidovorax, Afipia, Brevundimonas, Propionibacterium, Serratia, and others were recovered in varying abundance. Retrieval of sequences arising from the iodine (biocide)-reducing Delftia acidovorans in postflight waters is also of concern. Total microbial burdens of ISS potable waters were derived from data generated by an ATP-based enumeration procedure, with results ranging from 0 to 4.9 × 104 cells/ml. Regardless of innate biases in sample collection and analysis, such circumstantial evidence for the presence of viable, intact pathogenic cells should not be taken lightly. Implementation of new cultivation approaches and/or viability-based assays are requisite to confirm such an occurrence.
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Keywords: 16S rDNA; Bioburden; International Space Station; Molecular; Pathogen; Potable water

Document Type: Research Article

Affiliations: 1: *Biotechnology and Planetary Protection Group, Jet Propulsion Laboratory, California Institute of Technology, Pasadena, CA 2: †Bionetics Corp., Kennedy Space Center, FL 3: ‡Johnson Space Center, Houston, TX

Publication date: January 1, 2004

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  • Habitation, International Journal for Human Support Research, is designed to meet the needs of an emerging field of study necessitated by the need to develop new technologies to support human activities within controlled environments.
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