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A Study on the Perfusion Preservation, Resuscitation, and Transplantation of a Rat Heart Isolated for 96 Hours


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Krebs-Henseleit (KH) solution was used to fill the heart chamber of an isolated rat heart before it was immersed in perfluorocarbon (PFC), which is an inert fluid. A gas mixture (PCO2 = 150 hPa and PO2 = 850 hPa) was then aerated at a constant rate into the PFC solution, and the isolated heart was thereafter preserved for 96 h with KH solution perfused continuously at a rate of 0.1 ml/h from the aorta of the isolated heart through a cannula. After preservation, the preserved heart was heterotopically transplanted into the neck of a recipient rat and then it was resuscitated. Using this method for preserving mammalian organs, we attained reproducibility after perfusion preservation for 96 h.
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Keywords: Heterotrophic transplantation; Isolated rat heart; Perfluorocarbon (PFC); Perfusion preservation; Preservation; Resuscitation

Document Type: Research Article

Affiliations: 1: Kanagawa University Faculty of Science, Hiratsukashi, 259-1293, Japan. [email protected] 2: Kanagawa University Faculty of Science, Hiratsukashi, 259-1293, Japan

Publication date: May 1, 2009

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