Skip to main content
padlock icon - secure page this page is secure

Pancreas Preservation by the Two-Layer Method: Does it Have a Beneficial Effect Compared With Simple Preservation in University of Wisconsin Solution?

Notice

The full text article is temporarily unavailable.

We apologise for the inconvenience. Please try again later.

A large number of reports have shown that the two-layer method (TLM), which employs oxygenated perfluorochemical (PFC) and University of Wisconsin (UW) solution, is superior to simple cold storage in UW in islet transplantation. However, two recent large-scale studies showed no beneficial effect of TLM compared with UW storage in human islet transplantation. We reevaluated the effect of TLM by following three groups: group 1: UW simple storage; group 2: TLM performed by multiorgan procurement teams (not specialists of islet isolation); and group 3: TLM performed by specialists of islet isolation (Noguchi and Matsumoto). There were no significant differences between groups 1 and 2, whereas islet yields were significantly higher in group 3 compared with either group 1 or 2. Our data suggest that exact, complete performance of TLM could improve the outcome of islet isolation and transplantation. In this review, we describe the mechanisms of the TLM, the procedure of preoxygenated TLM, and the several possibilities for the reasons of the discrepancy.
No References
No Citations
No Supplementary Data
No Article Media
No Metrics

Keywords: Adenosine triphosphate; Islet isolation; Islet transplantation; Perfluorochemical; Two-layer method; University of Wisconsin solution

Document Type: Research Article

Affiliations: Baylor Institute for Immunology Research/Baylor All Saints Medical Center, Baylor Research Institute, Dallas, TX, USA. [email protected]

Publication date: May 1, 2009

  • Access Key
  • Free content
  • Partial Free content
  • New content
  • Open access content
  • Partial Open access content
  • Subscribed content
  • Partial Subscribed content
  • Free trial content
Cookie Policy
X
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more