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Identification of chromosomes in Thinopyrum intermedium and wheat Th. intermedium amphiploids based on multiplex oligonucleotide probes

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Synthesized oligonucleotides (oligos) can be used as effective probes similar to plasmid clones for chromosome identification in fluorescence in situ hybridization (FISH) analysis, making oligo FISH a simpler and more efficient molecular cytogenetic technique for studying plants. In this study, multiplex oligonucleotide probes, including pSc119.2-1, pAs1-4, (GAA)10, (AAC)6, and pTa71, were combined and used in FISH to identify chromosomes in common wheat, Thinopyrum intermedium, and a wheat – Th. intermedium amphiploid TE256-1. In comparison with general FISH probes, signals generated by the multiplex probes were more abundant, colorful, and characteristic. Combining the results of genomic in situ hybridization (GISH) with FISH, Th. intermedium chromosomes and alien chromosomes in TE256-1 could be classified and identified more precisely, especially the J- and J s -genome chromosomes. Moreover, based on the FISH results using multiplex probes, more structural variations in wheat chromosomes of TE256-1 were detected. The results indicated that multiplex oligo probes would have a wide range of application prospects in the creation and identification of wheat – Th. intermedium germplasms.
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Keywords: Thinopyrum intermedium; chromosome variation; hybridation in situ; in situ hybridization; multiplex probes; sondes en multiplexe; variation chromosomique

Document Type: Research Article

Affiliations: 1: College of Agronomy, Shandong Agriculture University, Tai’an, Shandong 271018, China. 2: State Key Laboratory of Crop Biology, Shandong Agriculture University, Tai’an, Shandong 271018, China. 3: United States Department of Agriculture – Agricultural Research Services, Forage and Range Research Laboratory, Logan, UT 84322-6300, USA.

Publication date: January 1, 2018

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