Provider: Ingenta Connect Database: Ingenta Connect Content: application/x-research-info-systems TY - ABST AU - Sun, Hong-Zhao AU - Zhao, Shu-Zhen AU - Ai, Hong-Bin TI - Microinjection of l-glutamate into the nucleus ambiguus partially inhibits gastric motility through the NMDA receptor Рnitric oxide pathway JO - Canadian Journal of Physiology and Pharmacology PY - 2014-01-01T00:00:00/// VL - 92 IS - 6 SP - 455 EP - 459 KW - nucleus ambiguus KW - oxyde nitrique KW - r̩cepteur NMDA KW - gastric motility KW - L-glutamate KW - motilit̩ gastrique KW - NMDA receptor KW - nitric oxide KW - l-glutamate KW - noyau ambigu N2 - We have previously reported that both l-glutamate (l-Glu) and nitric oxide (NO) modulate gastric motility in the nucleus ambiguus (NA). The aim of this study is to explore the potential correlation between the l-Glu and NO. A latex balloon connected to a pressure transducer was inserted into the pylorus through the fundus of anesthetized male Wistar rats to continuously record changes in gastric smooth muscle contractile curves. Pretreatment with the NO-synthase inhibitor N-nitro-l-arginine methylester (l-NAME) did not completely abolish the inhibitory effect of l-Glu on gastric motility, but intravenous injection of the ganglionic blocker hexamethonium bromide (Hb) did. By using a specific N-methyl-d-aspartic acid (NMDA) receptor antagonist, we blocked the inhibitory effect of the NO-donor sodium nitroprusside (SNP) on gastric motility. These results suggest that microinjections of l-Glu into the NA inhibits gastric motility by activating the cholinergic preganglionic neurons, partially through the NMDA receptor РNO pathway. UR - https://www.ingentaconnect.com/content/cndscipub/cjpp/2014/00000092/00000006/art00005 M3 - doi:10.1139/cjpp-2013-0413 UR - https://doi.org/10.1139/cjpp-2013-0413 ER -