Microinjection of l-glutamate into the nucleus ambiguus partially inhibits gastric motility through the NMDA receptor – nitric oxide pathway
We have previously reported that both l-glutamate (l-Glu) and nitric oxide (NO) modulate gastric motility in the nucleus ambiguus (NA). The aim of this study is to explore the potential correlation between the l-Glu and NO. A latex balloon connected to a pressure transducer was inserted
into the pylorus through the fundus of anesthetized male Wistar rats to continuously record changes in gastric smooth muscle contractile curves. Pretreatment with the NO-synthase inhibitor N-nitro-l-arginine methylester (l-NAME) did not completely abolish the inhibitory effect of l-Glu
on gastric motility, but intravenous injection of the ganglionic blocker hexamethonium bromide (Hb) did. By using a specific N-methyl-d-aspartic acid (NMDA) receptor antagonist, we blocked the inhibitory effect of the NO-donor sodium nitroprusside (SNP) on gastric motility. These results
suggest that microinjections of l-Glu into the NA inhibits gastric motility by activating the cholinergic preganglionic neurons, partially through the NMDA receptor – NO pathway.
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Document Type: Research Article
College of Life Science, Qi Lu Normal University, No. 36, Lishan Road, Jinan, Shandong 250013, P.R. China.
Biotechnology Research Center, Shandong Academy of Agricultural Sciences, Jinan, Shandong 250100, P.R. China.
Key Laboratory of Animal Resistance, College of Life Science, Shandong Normal University, No. 88, East Wenhua Road, Jinan, Shandong 250014, P.R. China.
Publication date: January 1, 2014
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