The aim of this study was to investigate the hepatoprotective role of azadirachtin-A in carbon tetrachloride (CCl4) induced hepatotoxicity in rats. The group allotment for the animals used in the hepatoprotective study included a vehicle treatment group, CCl4 (1
mL·(kg body mass)–1) treatment group, silymarin (100 μg·(kg body mass)–1·day–1) + CCl4 treatment group, and groups treated with different doses of azadirachtin-A (100 or 200 μg·(kg body mass)–1·day–1)
+ CCl4. On the 9th day, blood was obtained for measuring the biochemical parameters, and liver tissue was obtained for pathological examination. The acute toxicity test with azadirachtin-A (500, 1000, or 2000 μg·(kg body mass)−1) indicated no mortality
after 14 days of treatment; further, there was no change in behavior, food consumption, or organ mass. However with the higher dose, some hematological parameters showed changes. Hepatoprotective studies revealed that the CCl4 treatment group exhibited a decrease in total protein
and albumin levels, whereas a significant increase in BUN, AST, ALT, and ALP levels were noticed compared with the vehicle-treated control, indicating that there was liver damage caused by CCl4. Histology and ultrastructure study confirmed that pretreatment with azadirachtin-A dose-dependently
reduced hepatocellular necrosis and, therefore, protected the liver against toxicity caused by CCl4. The results from this study indicate that pretreatment with azadirachtin-A at the higher dose levels, moderately restores the rat liver to normal. This study confirms that azadirachtin-A
possesses greater hepatoprotective action; however, the effective concentration needs to be determined.
No Reference information available - sign in for access.
No Citation information available - sign in for access.
No Supplementary Data.
No Article Media
la toxicité aiguë;
Document Type: Research Article
KLES Kidney Foundation, KLES Prabhakar Kore Hospital & MRC, Belgaum 590 010, Karnataka, India.
Central Animal Facility, Indian Institute of Science, Bengaluru 560012, Karnataka, India.
Department of Zoology, College of Science, King Saud University, PO Box 2455, Riyadh 11451, Kingdom of Saudi Arabia.
Publication date: January 1, 2014
More about this publication?