Bacteriophages are associated with reduced fecal shedding of Shiga-toxin-producing Escherichia coli O157:H7 (STEC O157:H7) in cattle. Four phages exhibiting activity against 12 of 14 STEC O157:H7
strains, representing 11 common phage types, were isolated. Phages did not lyse non-O157 E. coli, with 11 of the 12 STEC strains exhibiting extreme susceptibility (average multiplicity of infection (MOI) = 0.0003−0.0007). All phages had icosahedral heads with tapered, noncontractile
tails, a morphology indicative of T1-like Siphoviridae. Genome size of all phages was ∼44 kb, but EcoRІ or HindIII digestion profiles differed among phages. Based on restriction enzyme digestion profiles, phages AHP24, AHS24, and AHP42 were more related
(66.7%−82.4%) to each other than to AKS96, while AHP24 and AHS24, isolated from the same feedlot pen, exhibited the highest identity (88.9%−92.3%). Phages AHP24 and AHS24 exhibited the broadest host range and strongest lytic activity against STEC O157:H7, making them strong candidates
for biocontrol of this bacterium in cattle.
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Escherichia coli O157:H7;
Document Type: Research Article
Lethbridge Research Centre, Agriculture and Agri-Food Canada, Lethbridge, AB T1J 4B1, Canada.
Alberta Agriculture and Rural Development, Agriculture Centre, Lethbridge, AB T1J 4V6, Canada.
Département de microbiologie, Faculté de médecine, Université Laval, Québec, QC G1K 7P4, Canada.
Publication date: July 12, 2012
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