Provider: Ingenta Connect Database: Ingenta Connect Content: application/x-research-info-systems TY - ABST AU - Dai AU - Li AU - Wang AU - Huang TI - Aberrant promoter methylation reduced the expression of protocadherin 17 in nasopharyngeal cancer JO - Biochemistry and Cell Biology PY - 2019-01-01T00:00:00/// VL - 97 IS - 4 SP - 364 EP - 368 KW - nasopharyngeal cancer KW - methylation KW - prolifération KW - apoptosis KW - apoptose KW - proliferation KW - méthylation KW - cancer nasopharyngien N2 - This study aimed to explore the underlying mechanism of protocadherin 17 (PCDH17) downregulated in nasopharyngeal cancer (NPC). NPC tumor and adjacent tissue samples were collected from NPC patients who received therapy in the Chinese PLA General Hospital. Meanwhile, a normal epithelial cell lines NP96 and 6 NPC and cell lines C666-1, CEN1, CEN2, HNE1, HEN2, and HONE1 were prepared. Then, the expression level of PCDH17 and the methylation level of PCDH17 promoter in both tissues samples and cell lines were determined using the PCR method. Moreover, PCDH17 was overexpressed in CNE2 and HONE1 using Lipo2000. Following this, the proliferation and apoptosis of CNE2 and HONE1 were assessed using MTT and flow cytometry. The expression of PCDH17 was significantly downregulated in NPC tissues compared with the adjacent tissues as well as in the NPC cell lines compared with the normal NP96 cells. Overexpressed PCDH17 could significantly inhibit the proliferation of CNE2 and HONE1 cells but obviously promote the apoptosis of these two cell lines. Aberrant hypermethylation in the promoter might be the explanation of PCDH17 downregulated in PCDH17 and promoted the development of NPC. UR - https://www.ingentaconnect.com/content/cndscipub/bcb/2019/00000097/00000004/art00003 M3 - doi:10.1139/bcb-2017-0343 UR - https://doi.org/10.1139/bcb-2017-0343 ER -