Skip to main content
padlock icon - secure page this page is secure

Chicken biliary exosomes enhance CD4+T proliferation and inhibit ALV-J replication in liver

Buy Article:

$36.22 + tax (Refund Policy)

Exosomes, which are small membrane vesicles of endocytic origin, carry lipids, RNA/miRNAs, and proteins and have immune modulatory functions. In this study, we isolated exosomes from the bile of specific pathogen-free chickens, 42–43 days of age, by using an ultracentrifugation and filtration method. The density of the exosomes, isolated by sucrose gradient fractionation, was between 1.13 and 1.19 g/mL. Electron microscopic observation of the liver showed that exosomes were present in the space of Disse and bile canaliculus. Chicken biliary exosomes displayed typical saucer-shaped, rounded morphology. Using liquid chromatography mass spectrum methodology, 196 proteins, including exosomal markers and several unique proteins, were identified and compared with mouse biliary exosomes. Noteworthy, CCCH type zinc finger antiviral protein was found on chicken biliary exosomes never described before. Furthermore, our data show that chicken biliary exosomes promote the proliferation of CD4+ and CD8+ T cells and monocytes from liver. In addition, chicken biliary exosomes significantly inhibit avian leukosis virus subgroup J, which is an oncogenic retrovirus, from replicating in the DF-1 cell line. These data indicate that chicken biliary exosomes possess the capacity to influence the immune responses of lymphocytes and inhibit avian leukosis virus subgroup J (ALV-J).
No Reference information available - sign in for access.
No Citation information available - sign in for access.
No Supplementary Data.
No Article Media
No Metrics

Keywords: ALV-J; CD4+T; VLA-J; cellules T CD4+; chicken biliary exosomes; exosomes biliaires de poulet; foie; liver; protéine antivirale à doigt de zinc; zinc finger antiviral protein

Document Type: Research Article

Affiliations: 1: College of Veterinary Medicine, Shandong Agricultural University, No. 61, Daizong ST, Tai’an 271018, P.R. China. 2: Department of Microbiology & Immunology, 309 Translational Research Building Brown Cancer Center, Louisville, KY, USA.

Publication date: January 1, 2014

More about this publication?
  • Access Key
  • Free content
  • Partial Free content
  • New content
  • Open access content
  • Partial Open access content
  • Subscribed content
  • Partial Subscribed content
  • Free trial content
Cookie Policy
X
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more