@article {Wimalarathna:2014:0829-8211:69,
title = "Co-dependent recruitment of Ino80p and Snf2p is required for yeast CUP1 activation",
journal = "Biochemistry and Cell Biology",
parent_itemid = "infobike://cndscipub/bcb",
publishercode ="cndscipub",
year = "2014",
volume = "92",
number = "1",
publication date ="2014-01-01T00:00:00",
pages = "69-75",
itemtype = "ARTICLE",
issn = "0829-8211",
eissn = "1208-6002",
url = "https://www.ingentaconnect.com/content/cndscipub/bcb/2014/00000092/00000001/art00008",
doi = "doi:10.1139/bcb-2013-0097",
keyword = "homéostasie du cuivre, remodelage de la chromatine, INO80, copper homeostasis, SWI/SNF, chromatin remodeling, CUP1",
author = "Wimalarathna, Roshini N. and Pan, Po Yun and Shen, Chang-Hui",
abstract = "In yeast, Ace1p-dependent induction of CUP1 is responsible for protecting cells from copper toxicity. Although the mechanism of yeast CUP1 induction has been studied intensively, it is still uncertain which chromatin remodelers are involved in CUP1 transcriptional
activation. Here, we show that yeast cells are inviable in the presence of copper when either chromatin remodeler, Ino80p or Snf2p, is not present. This inviability is due to the lack of CUP1 expression in ino80 and snf2 cells. Subsequently, we observe that both
Ino80p and Snf2p are present at the promoter and they are responsible for recruiting chromatin remodeling activity to the CUP1 promoter under induced conditions. These results suggest that they directly participate in CUP1 transcriptional activation. Furthermore, the codependent
recruitment of both INO80 and SWI/SNF depends on the presence of the transcriptional activator, Ace1p. We also demonstrate that both remodelers are required to recruit RNA polymerase II and targeted histone acetylation, indicating that remodelers are recruited to the CUP1 promoter before
RNA polymerase II and histone acetylases. These observations provide evidence for the mechanism of CUP1 induction. As such, we propose a model that describes novel insight into the order of events in CUP1 activation.",
}