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Characterization of the O-antigen in the lipopolysaccharide of Cronobacter (Enterobacter) malonaticus 3267

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Cronobacter malonaticus, a Gram-negative bacterium previously known as Enterobacter sakazakii, is an opportunistic pathogen known to cause serious infection in infants and neonates. To provide aid for the serological and chemical identification of clinical, environmental, or food isolates of this emerging pathogen, the characterization of the lipopolysaccharide (LPS) O-polysaccharide (O-PS) antigens of Cronobacter spp. is being undertaken. The structural analysis of the O-PS, obtained by hydrazinolysis of the lipopolysaccharide produced by Cronobacter malonaticus HPB 3267, was investigated by composition, methylation, and two-dimensional high-resolution nuclear magnetic resonance methods, and was found to be a polymer of a branched pentasaccharide unit. This unit is composed of d-glucose (d-Glc), d-galactose (d-Gal), 2-amino-2-deoxy-d-glucose (d-GlcN), 2-amino-2-deoxy-d-galactose (d-GalN) and 3-deoxy-d-manno-oct-2-ulosonic acid (Kdo) residues (1: 1: 1: 1: 1), forms the repeating oligosaccharide in the O-PS antigen, and has the structure:

Cronobacter malonaticus, une bactérie à Gram négatif, connue précédemment sous le nom de Enterobacter sakazakii, est un pathogène opportuniste qui cause des infections graves chez le bébé et le nouveau-né. Afin d’aider à l’identification sérologique et chimique des isolats cliniques, environnementaux ou alimentaires de ce pathogène en émergence, la caractérisation du LPS O-polysaccharide antigénique de Cronobacter spp. a été entreprise. La structure de l’O-PS obtenu après hydrazinolyse du lipopolysaccharide produit par la souche Cronobacter malonaticus HPB 3267 a été examinée par composition, méthylation et résonance magnétique nucléaire 2D à haute résolution. Il consiste en un polymère de penta-saccharides branchés. L’unité est composée de d-glucose (d-Glc), de d-galactose (d-Gal), de 2-amino-2-déoxy-d-glucose (d-GlcN), de 2-amino-2-déoxy-d-galactose (d-GalN) et d’acide 3-déoxy-d-manno-oct-2-ulosonique (Kdo) (1 :1 :1 :1 :1), et elle forme l’oligosaccharide répété constituant l’O-PS et possède la structure suivante :
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Document Type: Research Article

Publication date: December 1, 2009

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