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Survey of bacterial microorganisms in the conjunctival sac of clinically normal dogs and dogs with ulcerative keratitis in Fortaleza, Ceará, Brazil

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Abstract Objective 

The ocular microflora in dogs has not been established in north-east Brazil. Thus, the main aim of this research was to determine the bacterial microorganisms in the conjunctival sac of clinically normal dogs and dogs with ulcerative keratitis in Fortaleza, Ceará, Brazil. Animals studied 

This study included 60 healthy dogs, 15 dogs with unilateral corneal ulcer, and three dogs with bilateral corneal ulcers. Procedure 

Samples were taken by a calibrated platinum loop (1 µL) placed directly onto the conjunctival sac and on sterile blood agar. The clinical specimens were incubated at 37 °C in an atmosphere of 5% CO2 for 48 h. Results 

Of the 120 samples from healthy dogs, only 47 (39%) had positive culture for bacteria, while all of the specimens from eyes with corneal ulcer were positive for bacterial growth. The group of dogs with corneal ulcer had a higher (P < 0.05) number of colony-forming units (CFU) per plate than the group of healthy animals. Of the 59 isolates from healthy eyes, only nine (15.3%) had more than 50 CFU per plate, while in the group of dogs with corneal ulcer, 23 (62.2%) of the 37 isolates presented more than 50 CFU per plate. In both groups Gram-positive bacteria (86.5%) predominated over Gram-negative (13.5%). Staphylococcus spp. was the most frequently isolated genus and S. intermedius predominated in both groups. Conclusion 

The results of our study are directly applicable to initiate rational, preventive and therapeutic measures with greater accuracy in dogs with corneal ulcer.
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Keywords: bacteria; conjunctival sac; corneal ulcer; dog

Document Type: Research Article

Affiliations: 1: School of Veterinary Medicine, Post-Graduation Program in Veterinary Science, State University of Ceará, Fortaleza − CE, Brazil; 2: Department of Statistics and Applied Mathematics, Federal University of Ceará, Fortaleza − CE, Brazil 3: Department of Pathology and Forensic Medicine, School of Medicine, Medical Mycology Specialized Center, Federal University of Ceará, Fortaleza − CE, Brazil;

Publication date: January 1, 2005

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