Skip to main content
padlock icon - secure page this page is secure

In vitro effects of CpG oligodeoxynucleotides delivered by gelatin nanoparticles on canine peripheral blood mononuclear cells of atopic and healthy dogs – a pilot study

Buy Article:

$52.00 + tax (Refund Policy)

Background

Cytosine‐phosphate‐guanine (CpG) oligodeoxynucleotides offer a novel promising immunotherapeutic approach for atopic dermatitis (AD) both in humans and animals. Gelatin nanoparticles (GNP) enhance and prolong CpG‐associated immunomodulatory effects and minimize adverse effects both in vitro and in vivo. Information about the effects of this combination in dogs is lacking.
Hypothesis/Objectives

The aim of this study was to evaluate immunological effects of CpG coupled to GNP on canine peripheral blood mononuclear cells (PBMCs) in vitro.
Animals

Eight dogs with AD, diagnosed by standard criteria and with a concurrent immediate hypersensitivity to house dust mites were included. Control samples were taken from eight healthy, age‐matched control dogs without history or evidence of cutaneous or systemic illness.
Methods

Peripheral blood mononuclear cells of healthy and allergic dogs were incubated with CpG‐GNP and the uptake of CpG‐GNP was demonstrated using confocal laser scanning microscopy. Cell culture supernatant concentrations of interferon gamma (IFN‐γ), interleukin (IL)‐4, IL‐6 and IL‐10 were measured by Canine Cytokine Milliplex.
Results

No significant changes in IFN‐γ and IL‐4 were found when comparing PBMCs incubated with CpG and CpG‐GNP with the negative controls in atopic and healthy dogs. Interleukin‐6 was not detected in any of the groups. However, a statistically significant increase in IL‐10 concentration was found after 24 h stimulation with CpG‐GNP compared with CpG alone both in atopic and healthy dogs.
Conclusions and clinical importance

As IL‐10 is considered an immunosuppressive cytokine playing a key role in peripheral tolerance; the reported CpG‐GNP formulation could be a new approach in allergy treatment.
No References
No Citations
No Supplementary Data
No Article Media
No Metrics

Language: French

Document Type: Research Article

Publication date: October 1, 2013

  • Access Key
  • Free content
  • Partial Free content
  • New content
  • Open access content
  • Partial Open access content
  • Subscribed content
  • Partial Subscribed content
  • Free trial content
Cookie Policy
X
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more