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A rabbit model for monitoring in vivo viability of human platelet concentrates using flow cytometry

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BACKGROUND: Viability in vivo of novel platelet components cannot be readily determined in human transfusions. Elaboration of valid animal models may be useful for this purpose.

STUDY DESIGN AND METHODS: Viability of platelet concentrates (PCs) WBC reduced before storage was determined by flow cytometry in rabbits whose reticuloendothelial system was inhibited by ethyl palmitate administration. PCs stored at 22°C for 2 and 5 days (D2- and D5-PCs) or refrigerated PCs (3-6 days at 22°C plus 1-4 days at 4°C, RF-PCs) were transfused into rabbits. Five parameters of PC viability in vivo were calculated from human platelet survival curves: survival time, recovery 0.5 and 24 hours after transfusion (R0.5, R24), maximal recovery (Rmax), and total recovery for 0 to 24 hours (RΣ).

RESULTS: No differences in viability of D2- and D5-PCs were found. In contrast, viability of RF-PCs was significantly lower than that of D2-PCs, as was revealed with diverse sensitivity by four parameters: RΣ > R24 > R0.5 = survival time (p < 0.001, p < 0.01, and p < 0.05, respectively).

CONCLUSION: The rabbit model elaborated is sufficiently sensitive to reveal differences in human platelet viability in vivo between conventional and cold-damaged PCs. It may be useful for comparing viability of different platelet components that cannot be readily tested in human transfusions.
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Document Type: Research Article

Affiliations: From the Department of Transfusion Medicine at the St. Michael's Hospital;

Publication date: 01 June 2002

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