@article {Gamir:2018:0960-7412:518,
title = "Starch degradation, abscisic acid and vesicular trafficking are important elements in callose priming by indole3carboxylic acid in response to Plectosphaerella cucumerina infection",
journal = "The Plant Journal",
parent_itemid = "infobike://bsc/tpj",
publishercode ="bp",
year = "2018",
volume = "96",
number = "3",
publication date ="2018-11-01T00:00:00",
pages = "518-531",
itemtype = "ARTICLE",
issn = "0960-7412",
eissn = "1365-313X",
url = "https://www.ingentaconnect.com/content/bsc/tpj/2018/00000096/00000003/art00004",
doi = "doi:10.1111/tpj.14045",
keyword = "defence priming, BAM1, Plectosphaerella cucumerina, indole‐3‐carboxylic acid, callose accumulation",
author = "Gamir, Jordi and Pastor, Victoria and S{\’a}nchezBel, Paloma and Agut, Blas and Mateu, Diego and Garc{\’ı}aAndrade, Javier and Flors, V{\’ı}ctor",
abstract = "A fast callose accumulation has been shown to mediate defence priming in certain plantpathogen interactions, but the events upstream of callose assembly following chemical priming are poorly understood, mainly because those steps comprise sugar transfer to the infection site.
Amino butyric acid (BABA)induced resistance in Arabidopsis against Plectosphaerella cucumerina is known to be mediated by callose priming. Indole3carboxylic acid (ICOOH, also known as I3CA) mediates BABAinduced resistance in Arabidopsis
against P.cucumerina. This indolic compound is found in a common fingerprint of primed metabolites following treatments with various priming stimuli. In the present study, we show that I3CA induces resistance in Arabidopsis against P.cucumerina and primes enhancement
of callose accumulation. I3CA treatment increased abscisic acid (ABA) levels before infection with P.cucumerina. An intact ABA synthesis pathway is needed to activate a starch amylase (BAM1) to trigger augmented callose deposition against P.cucumerina during
I3CAIR. To verify the relevance of the BAM1 amylase in I3CAIR, knockdown mutants and overexpressors of the BAM1 gene were tested. The mutant bam1 was impaired to express I3CAIR, but complemented 35S::BAM1YFP lines in the background of bam1
restored an intact I3CAIR and callose priming. Therefore, a more active starch metabolism is a committed step for I3CAIR, inducing callose priming in adult plants. Additionally, I3CA treatments induced expression of the ubiquitin ligase ATL31 and syntaxin SYP131,
suggesting that vesicular trafficking is relevant for callose priming. As a final element in the callose priming, an intact Powdery Mildew resistant4 (PMR4) gene is also essential to fully express I3CAIR.",
}