Skip to main content
padlock icon - secure page this page is secure

Free Content Establishment of genetically encoded biosensors for cytosolic boric acid in plant cells

Download Article:
 Download
(PDF)
 
Boron (B) is an essential micronutrient for plants. To maintain B concentration in tissues at appropriate levels, plants use boric acid channels belonging to the NIP subfamily of aquaporins and BOR borate exporters. To regulate B transport, these transporters exhibit different cell‐type specific expression, polar localization, and B‐dependent post‐transcriptional regulation. Here, we describe the development of genetically encoded biosensors for cytosolic boric acid to visualize the spatial distribution and temporal dynamics of B in plant tissues. The biosensors were designed based on the function of the NIP5;1 5′‐untranslated region (UTR), which promotes mRNA degradation in response to an elevated cytosolic boric acid concentration. The signal intensities of the biosensor coupled with Venus fluorescent protein and a nuclear localization signal (uNIP5;1‐Venus) showed negative correlation with intracellular B concentrations in cultured tobacco BY‐2 cells. When expressed in Arabidopsis thaliana, uNIP5;1‐Venus enabled the quantification of B distribution in roots at single‐cell resolution. In mature roots, cytosolic B levels in stele were maintained under low B supply, while those in epidermal, cortical, and endodermal cells were influenced by external B concentrations. Another biosensor coupled with a luciferase protein fused to a destabilization PEST sequence (uNIP5;1‐Luc) was used to visualize changes in cytosolic boric acid concentrations. Thus, uNIP5;1‐Venus/Luc enables visualization of B transport in various plant cells/tissues.
No References
No Citations
No Supplementary Data
No Article Media
No Metrics

Keywords: 5′UTR; Arabidopsis thaliana; BY‐2; Nicotiana tabacum; biosensor; boric acid; boron; channel; mRNA degradation; transporter

Document Type: Research Article

Publication date: September 1, 2018

  • Access Key
  • Free content
  • Partial Free content
  • New content
  • Open access content
  • Partial Open access content
  • Subscribed content
  • Partial Subscribed content
  • Free trial content
Cookie Policy
X
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more