Skip to main content
padlock icon - secure page this page is secure

Free Content GBF1 differentially regulates CAT2 and PAD4 transcription to promote pathogen defense in Arabidopsis thaliana

Download Article:
 Download
(PDF)
 
G‐BOX BINDING FACTOR 1 (GBF1) influences light‐regulated seedling development in Arabidopsis, and inhibits CATALASE 2 (CAT2) expression during senescence. CAT2 functions as a scavenger of hydrogen peroxide. The role of GBF1 in the defense response is not known. We report here that GBF1 positively influences the defense against virulent and avirulent strains of Pseudomonas syringae. The gbf1 mutants are susceptible, whereas GBF1 over‐expresser transgenic plants are resistant to bacterial pathogens. GBF1 negatively regulates pathogen‐induced CAT2 expression and thereby positively regulates the hypersensitive response. In addition to CAT2 promoter, GBF1 binds to the G‐box‐like element present in the intron of PHYTOALEXIN DEFICIENT 4 (PAD4). This association of GBF1 with PAD4 intron is enhanced upon pathogenesis. GBF1 positively regulates PAD4 transcription in an intron‐dependent manner. GBF1‐mediated positive regulation of PAD4 expression is also evident in gbf1 mutant and GBF1 over‐expression lines. Similar to pad4 mutants, pathogen‐induced camalexin and salicylic acid (SA) accumulation, and expression of SA‐inducible PATHOGENESIS RELATED1 (PR1) gene are compromised in the gbf1 mutant. Exogenous application of SA rescues the loss‐of‐defense phenotypes of gbf1 mutant. Thus, altogether, our results demonstrate that GBF1 is an important component of the plant defense response that functions upstream of SA accumulation and, by oppositely regulating CAT2 and PAD4, promotes disease resistance in Arabidopsis.
No References
No Citations
No Supplementary Data
No Article Media
No Metrics

Keywords: Arabidopsis thaliana; Botrytis cinerea; CAT2; GBF1; PAD4; Pseudomonas syringae; compatible interaction; hypersensitive response; incompatible interaction

Document Type: Research Article

Publication date: September 1, 2017

  • Access Key
  • Free content
  • Partial Free content
  • New content
  • Open access content
  • Partial Open access content
  • Subscribed content
  • Partial Subscribed content
  • Free trial content
Cookie Policy
X
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more