Skip to main content
padlock icon - secure page this page is secure

Free Content Nitrogen starvation‐induced accumulation of triacylglycerol in the green algae: evidence for a role for ROC40, a transcription factor involved in circadian rhythm

Download Article:

You have access to the full text article on a website external to Ingenta Connect.

Please click here to view this article on Wiley Online Library.

You may be required to register and activate access on Wiley Online Library before you can obtain the full text. If you have any queries please visit Wiley Online Library

Microalgal triacylglycerol (TAG), a promising source of biofuel, is induced upon nitrogen starvation (−N), but the proteins and genes involved in this process are poorly known. We performed isobaric tagging for relative and absolute quantification (iTRAQ)‐based quantitative proteomics to identify Chlorella proteins with modulated expression under short‐term −N. Out of 1736 soluble proteins and 2187 membrane‐associated proteins identified, 288 and 56, respectively, were differentially expressed under −N. Gene expression analysis on select genes confirmed the same direction of mRNA modulation for most proteins. The MYB‐related transcription factor ROC40 was the most induced protein, with a 9.6‐fold increase upon −N. In a previously generated Chlamydomonas mutant, gravimetric measurements of crude total lipids revealed that roc40 was impaired in its ability to increase the accumulation of TAG upon −N, and this phenotype was complemented when wild‐type Roc40 was expressed. Results from radiotracer experiments were consistent with the roc40 mutant being comparable to the wild type in recycling membrane lipids to TAG but being impaired in additional de novo synthesis of TAG during −N stress. In this study we provide evidence to support the hypothesis that transcription factor ROC40 has a role in −N‐induced lipid accumulation, and uncover multiple previously unknown proteins modulated by short‐term −N in green algae.
No References
No Citations
No Supplementary Data
No Article Media
No Metrics

Keywords: Chlamydomonas; Chlorella spp; algae; nitrogen starvation; proteomics; triacylglycerol

Document Type: Research Article

Publication date: March 1, 2016

  • Access Key
  • Free content
  • Partial Free content
  • New content
  • Open access content
  • Partial Open access content
  • Subscribed content
  • Partial Subscribed content
  • Free trial content
Cookie Policy
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more