@article {Figueroa:2015:0960-7412:849,
title = "Male sterility in Arabidopsis induced by overexpression of a MYC5SRDX chimeric repressor",
journal = "The Plant Journal",
parent_itemid = "infobike://bsc/tpj",
publishercode ="bp",
year = "2015",
volume = "81",
number = "6",
publication date ="2015-03-01T00:00:00",
pages = "849-860",
itemtype = "ARTICLE",
issn = "0960-7412",
eissn = "1365-313X",
url = "https://www.ingentaconnect.com/content/bsc/tpj/2015/00000081/00000006/art00002",
doi = "doi:10.1111/tpj.12776",
keyword = "stamen, Arabidopsis thaliana, MYC5, jasmonate, sterile, signaling",
author = "Figueroa, Pablo and Browse, John",
abstract = "Jasmonate hormone (JA) plays critical roles in both plant defense and reproductive development. Arabidopsis thaliana plants deficient in JAbiosynthesis or signaling are malesterile, with defects in stamen and pollen development. MYC2, MYC3 and MYC4 are
JAZinteracting bHLH transcription factors that play a major role in controlling JA responses in vegetative tissue, but are not likely to play a role in reproductive tissue. We found that a closely related transcription factor, MYC5 (bHLH28), was able to induce JAZ promoters
that control some of the early JAresponsive genes in a Daucus carota (carrot) protoplast expression system. A Gbox sequence in the JAZ2 promoter was necessary and sufficient for induction by MYC5 (as it is for MYC2, MYC3 and MYC4), and induction of JAZ
genes was repressed by coexpression of a stabilized, JAZ1Jas repressor. Two allelic myc5 mutants exhibited no overt phenotype; however, transgenic lines expressing MYC5 fused to an SRDX (SUPERMAN repressive domainX) motif phenocopied mutants defective in JA signaling.
In particular, MYC5SRDX plants were malesterile, with defects in stamen filament elongation, anther dehiscence and pollen viability. Importantly, expression of MYB21 and other transcription factors required for stamen and pollen maturation was strongly reduced in stamens of
MYC5SRDX plants relative to the wild type. Taken together, these results indicate that MYC5, probably together with other, redundant transcription factors, may be activated by JA signaling to induce the expression of MYB21 and components required for male fertility.",
}