Skip to main content
padlock icon - secure page this page is secure

Free Content Changes in genome content generated via segregation of non‐allelic homologs

Download Article:

You have access to the full text article on a website external to Ingenta Connect.

Please click here to view this article on Wiley Online Library.

You may be required to register and activate access on Wiley Online Library before you can obtain the full text. If you have any queries please visit Wiley Online Library


A careful analysis of two maize recombinant inbred lines (RILs) relative to their inbred parents revealed the presence of several hundred apparently de novo copy number variants (CNVs). These changes in genome content were validated via both PCR and whole exome‐array capture‐and‐sequencing experiments. One hundred and eighty‐five genomic regions, which overlap with 38 high‐confidence genes, exhibited apparently de novo copy number variation (CNV) in these two RILs and in many instances the same apparently de novo CNV events were observed in multiple RILs. Further analyses revealed that these recurrent apparently de novo CNVs were caused by segregation of single‐copy homologous sequences that are located in non‐allelic positions in the two parental inbred lines. F1 individuals derived from these inbred lines will be hemizygous for each of these non‐allelic homologs but RIL genotypes will contain these sequences at zero, one or two genomic loci. Hence, the segregation of non‐allelic homologs may contribute to transgressive segregation. Indeed, statistical associations between phenotypic quantitative trait loci and genomic losses were observed for two of 14 tested pairs of non‐allelic homologs.
No References
No Citations
No Supplementary Data
No Article Media
No Metrics

Document Type: Research Article

Affiliations: 1: Department of Agronomy, 2035 Roy J. Carver Co-Lab, Iowa State University, Ames, IA 50011, USA 2: Interdepartmental Genetics, 2035 Roy J. Carver Co-Lab, Iowa State University, Ames, IA 50011, USA 3: Research Informatics, Roche NimbleGen, 500 South Rosa Road, Madison, WI 53705, USA 4: Development and Research, Roche NimbleGen, 500 South Rosa Road, Madison, WI 53705, USA 5: State Key Lab of Agrobiotechnology, China Agriculture University, Beijing, China 6: Department of Plant Biology, 250 Biological Science Center, St Paul, MN 55108, USA 7: Department of Statistics, 2115 Snedecor, Iowa State University, Ames, IA 50011, USA

Publication date: November 1, 2012

  • Access Key
  • Free content
  • Partial Free content
  • New content
  • Open access content
  • Partial Open access content
  • Subscribed content
  • Partial Subscribed content
  • Free trial content
Cookie Policy
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more