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Free Content HKT2;2/1, a K+‐permeable transporter identified in a salt‐tolerant rice cultivar through surveys of natural genetic polymorphism

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We have investigated OsHKT2;1 natural variation in a collection of 49 cultivars with different levels of salt tolerance and geographical origins. The effect of identified polymorphism on OsHKT2;1 activity was analysed through heterologous expression of variants in Xenopus oocytes. OsHKT2;1 appeared to be a highly conserved protein with only five possible amino acid substitutions that have no substantial effect on functional properties. Our study, however, also identified a new HKT isoform, No‐OsHKT2;2/1 in Nona Bokra, a highly salt‐tolerant cultivar. No‐OsHKT2;2/1 probably originated from a deletion in chromosome 6, producing a chimeric gene. Its 5′ region corresponds to that of OsHKT2;2, whose full‐length sequence is not present in Nipponbare but has been identified in Pokkali, a salt‐tolerant rice cultivar. Its 3′ region corresponds to that of OsHKT2;1. No‐OsHKT2;2/1 is essentially expressed in roots and displays a significant level of expression at high Na+ concentrations, in contrast to OsHKT2;1. Expressed in Xenopus oocytes or in Saccharomyces cerevisiae, No‐OsHKT2;2/1 exhibited a strong permeability to Na+ and K+, even at high external Na+ concentrations, like OsHKT2;2, and in contrast to OsHKT2;1. Our results suggest that No‐OsHKT2;2/1 can contribute to Nona Bokra salt tolerance by enabling root K+ uptake under saline conditions.
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Document Type: Research Article

Affiliations: 1: Biochimie et physiologie Moléculaire des Plantes, Institut de Biologie Intégrative des Plantes, UMR 5004 CNRS/UMR 0386 INRA/Montpellier SupAgro/Université Montpellier 2, 34060 Montpellier Cedex 2, France 2: Centro de Biotecnología y Genómica de Plantas, Universidad Politécnica de Madrid, Madrid, Campus de Montegancedo, Carretera M-40, km 37.7, 28223-Pozuelo de Alarcón, Madrid, Spain 3: Instituto Valenciano de Investigaciones Agrarias, Carretera Moncada – Náquera Km 4,5, Moncada-46113, Valencia, Spain 4: Biochimie et physiologie Moléculaire des Plantes, Institut de Biologie Intégrative des Plantes, UMR 5004 CNRS/UMR 0386 INRA/Montpellier SupAgro/Université Montpellier 2, 34060 Montpellier Cedex 2, France

Publication date: September 1, 2012

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