@article {Eberhard:2011:0960-7412:1055,
title = "Dual functions of the nucleusencoded factor TDA1 in trapping and translation activation of",
journal = "The Plant Journal",
parent_itemid = "infobike://bsc/tpj",
publishercode ="bp",
year = "2011",
volume = "67",
number = "6",
publication date ="2011-09-01T00:00:00",
pages = "1055-1066",
itemtype = "ARTICLE",
issn = "0960-7412",
eissn = "1365-313X",
url = "https://www.ingentaconnect.com/content/bsc/tpj/2011/00000067/00000006/art00010",
doi = "doi:10.1111/j.1365-313X.2011.04657.x",
author = "Eberhard, Stephan and Loiselay, Christelle and Drapier, Dominique and Bujaldon, Sandrine and GirardBascou, Jacqueline and Kuras, Richard and Choquet, Yves and Wollman, FrancisAndr{\’e}",
abstract = "Summary After endosymbiosis, organelles lost most of their initial genome. Moreover, expression of the few remaining genes became tightly controlled by the nucleus through transacting protein factors that are required for posttranscriptional
expression (maturation/stability or translation) of a single (or a few) specific organelle target mRNA(s). Here, we characterize the nucleusencoded TDA1 factor, which is specifically required for translation of the chloroplast atpA transcript that encodes subunit of
ATP synthase in Chlamydomonas reinhardtii. The sequence of TDA1 contains eight copies of a degenerate 38residue motif, that we named octotrico peptide repeat (OPR), which has been previously described in a few other transacting factors targeted to the C.reinhardtii
chloroplast. Interestingly, a proportion of the untranslated atpA transcripts are sequestered into highdensity, nonpolysomic, ribonucleoprotein complexes. Our results suggest that TDA1 has a dual function: (i) trapping a subset of untranslated atpA transcripts
into nonpolysomic complexes, and (ii) translational activation of these transcripts. We discuss these results in light of our previous observation that only a proportion of atpA transcripts are translated at any given time in the chloroplast of C.reinhardtii.
",
}