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Free Content Dual functions of the nucleus‐encoded factor TDA1 in trapping and translation activation of

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Summary

After endosymbiosis, organelles lost most of their initial genome. Moreover, expression of the few remaining genes became tightly controlled by the nucleus through trans‐acting protein factors that are required for post‐transcriptional expression (maturation/stability or translation) of a single (or a few) specific organelle target mRNA(s). Here, we characterize the nucleus‐encoded TDA1 factor, which is specifically required for translation of the chloroplast atpA transcript that encodes subunit α of ATP synthase in Chlamydomonas reinhardtii. The sequence of TDA1 contains eight copies of a degenerate 38‐residue motif, that we named octotrico peptide repeat (OPR), which has been previously described in a few other trans‐acting factors targeted to the C. reinhardtii chloroplast. Interestingly, a proportion of the untranslated atpA transcripts are sequestered into high‐density, non‐polysomic, ribonucleoprotein complexes. Our results suggest that TDA1 has a dual function: (i) trapping a subset of untranslated atpA transcripts into non‐polysomic complexes, and (ii) translational activation of these transcripts. We discuss these results in light of our previous observation that only a proportion of atpA transcripts are translated at any given time in the chloroplast of C. reinhardtii.
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Document Type: Research Article

Affiliations: 1: Unité Mixte de Recherche (UMR) 7141, Centre National de la Recherche Scientifique (CNRS) and Université Pierre et Marie Curie (UPMC - Paris 06), Institut de Biologie Physico-Chimique, 13 rue Pierre et Marie Curie, F-75005 Paris, France 2: Unité Mixte de Recherche (UMR) 7141, Centre National de la Recherche Scientifique (CNRS) and Université Pierre et Marie Curie (UPMC - Paris 06), Institut de Biologie Physico-Chimique, 13 rue Pierre et Marie Curie, F-75005 Paris, France 3: Unité Mixte de Recherche (UMR) 7141, Centre National de la Recherche Scientifique (CNRS) and Université Pierre et Marie Curie (UPMC - Paris 06), Institut de Biologie Physico-Chimique, 13 rue Pierre et Marie Curie, F-75005 Paris, France 4: Unité Mixte de Recherche (UMR) 7141, Centre National de la Recherche Scientifique (CNRS) and Université Pierre et Marie Curie (UPMC - Paris 06), Institut de Biologie Physico-Chimique, 13 rue Pierre et Marie Curie, F-75005 Paris, France

Publication date: September 1, 2011

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