Skip to main content
padlock icon - secure page this page is secure

Free Content A fast brassinolide-regulated response pathway in the plasma membrane of Arabidopsis thaliana

Download Article:

You have access to the full text article on a website external to Ingenta Connect.

Please click here to view this article on Wiley Online Library.

You may be required to register and activate access on Wiley Online Library before you can obtain the full text. If you have any queries please visit Wiley Online Library


To understand molecular processes in living plant cells, quantitative spectro-microscopic technologies are required. By combining fluorescence lifetime spectroscopy with confocal microscopy, we studied the subcellular properties and function of a GFP-tagged variant of the plasma membrane-bound brassinosteroid receptor BRI1 (BRI1–GFP) in living cells of Arabidopsis seedlings. Shortly after adding brassinolide, we observed BRI1-dependent cell-wall expansion, preceding cell elongation. In parallel, the fluorescence lifetime of BRI1–GFP decreased, indicating an alteration in the receptor’s physico-chemical environment. The parameter modulating the fluorescence lifetime of BRI1–GFP was found to be BL-induced hyperpolarization of the plasma membrane. Furthermore, for induction of hyperpolarization and cell-wall expansion, activation of the plasma membrane P-ATPase was necessary. This activation required BRI1 kinase activity, and was mediated by BL-modulated interaction of BRI1 with the P-ATPase. Our results were used to develop a model suggesting that there is a fast BL-regulated signal response pathway within the plasma membrane that links BRI1 with P-ATPase for the regulation of cell-wall expansion.
No References
No Citations
No Supplementary Data
No Article Media
No Metrics

Keywords: P-ATPase; brassinolide; brassinosteroid-insensitive 1 (BRI1); cell wall; fluorescence lifetime; membrane potential

Document Type: Research Article

Affiliations: 1: Laboratory of Plant Physiology and Biophysics, Faculty of Biomedical and Life Sciences – Bower Building, University of Glasgow, Glasgow G12 8QQ, UK 2: Center for Plant Molecular Biology, Department of Plant Physiology, University of Tübingen, Auf der Morgenstelle 1, 72076 Tübingen, Germany

Publication date: May 1, 2011

  • Access Key
  • Free content
  • Partial Free content
  • New content
  • Open access content
  • Partial Open access content
  • Subscribed content
  • Partial Subscribed content
  • Free trial content
Cookie Policy
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more