Skip to main content
padlock icon - secure page this page is secure

Free Content The preprophase band is a localized center of clathrin-mediated endocytosis in late prophase cells of the onion cotyledon epidermis

Download Article:

The preprophase band (PPB) marks the site on the plant cell cortex where the cell plate will fuse during the final stage of cytokinesis. Recent studies have shown that several cytoskeletal proteins are depleted at the PPB site, but the processes that bring about these changes are still unknown. We have investigated the membrane systems associated with the PPB regions of epidermal cells of onion cotyledons by means of serial thin sections and electron tomograms. In contrast with specimens preserved by chemical fixatives, our high-pressure frozen cells demonstrated the presence of large numbers of clathrin-coated pits and vesicles in the PPB regions. The vesicles were of two types: clathrin-coated and structurally related, non-coated vesicles. Quantitative analysis of the data revealed that the number of clathrin-coated pits and vesicles is higher in the PPB regions than outside of these regions. Immunofluorescent microscopy using anti-plant clathrin-antibody confirmed this result. In contrast, no differences in secretory activities were observed. We postulate that the removal of membrane proteins by endocytosis plays a role in the formation of PPB ‘memory’ structures.
No References
No Citations
No Supplementary Data
No Article Media
No Metrics

Keywords: clathrin-coated pits; clathrin-coated vesicles; electron tomography; endocytosis; preprophase band of microtubules

Document Type: Research Article

Affiliations: 1: Department of Biology, Graduate School of Science and Engineering, University of Toyama, Toyama 930-8555, Japan, 2: Department of Life Science, Graduate School of Life Science, University of Hyogo, 3-2-1 Kouto, Akou, Hyogo 678-1297, Japan, 3: RIKEN Center for Developmental Biology, 2-2-3 Minatojima-minamimachi, Chuou-ku, Kobe 650-0047, Japan, 4: MCD Biology, University of Colorado, Boulder, CO 80309-0347, USA,

Publication date: March 1, 2009

  • Access Key
  • Free content
  • Partial Free content
  • New content
  • Open access content
  • Partial Open access content
  • Subscribed content
  • Partial Subscribed content
  • Free trial content
Cookie Policy
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more