@article {Seltzer:2007:0960-7412:322,
title = "Arabidopsis GCP2 and GCP3 are part of a soluble -tubulin complex and have nuclear envelope targeting domains",
journal = "The Plant Journal",
parent_itemid = "infobike://bsc/tpj",
publishercode ="bp",
year = "2007",
volume = "52",
number = "2",
publication date ="2007-10-01T00:00:00",
pages = "322-331",
itemtype = "ARTICLE",
issn = "0960-7412",
eissn = "1365-313X",
url = "https://www.ingentaconnect.com/content/bsc/tpj/2007/00000052/00000002/art00010",
doi = "doi:10.1111/j.1365-313X.2007.03240.x",
keyword = "γ-tubulin complex protein 1, nucleation, microtubules, nuclear envelope, γ-tubulin complex protein",
author = "Seltzer, Virginie and Janski, Natacha and Canaday, Jean and Herzog, Etienne and Erhardt, Mathieu and Evrard, Jean-Luc and Schmit, Anne-Catherine",
abstract = "Summary In higher plants, microtubules (MTs) are assembled in distinctive arrays in the absence of a defined organizing center. Three MT nucleation sites have been described: the nuclear surface, the cell cortex and cortical MT branch points. The Arabidopsis thaliana (At) genome contains putative orthologues encoding all the components of characterized mammalian nucleation complexes: -tubulin and -tubulin complex proteins GCP2 to GCP6. We have cloned the cDNA encoding AtGCP2, and show that -tubulin, AtGCP2 and AtGCP3 are part of the same tandem affinity-purified complex and are present in a large membrane-associated complex. In addition, small soluble -tubulin complexes of the size expected for a -tubulin core complex are recruited to isolated nuclei. Using immunogold labelling, AtGCP3 is localized to both the nuclear envelope (NE) and the plasma membrane. To identify domains that could play a role in targeting complexes to these nucleation sites, truncated AtGCP2- and AtGCP3-green fluorescent protein fusion proteins were expressed in BY-2 cells. Several domains from AtGCP2 and AtGCP3 are capable of targeting fusions to the NE. We propose that regulated recruitment of soluble -tubulin-containing complexes is responsible for nucleation at dispersed sites in plant cells and contributes to the formation and organization of the various MT arrays.",
}