Skip to main content
padlock icon - secure page this page is secure

Free Content Plastid tRNA Genes trnC-GCA and trnN-GUU are essential for plant cell development

Download Article:
 Download
(PDF)
 
Summary

Higher plant chloroplast genomes code for a conserved set of 30 tRNAs. This set is believed to be sufficient to support translation, although import of cytosolic tRNA has been proposed to provide additional tRNA species to the chloroplast. Previous knock-outs of tRNA genes, or the pronounced reduction of the level of selected tRNAs, has not led to severe phenotypes. We deleted the two tRNA genes trnN-GUU and trnC-GCA independently from the plastid chromosome of tobacco. No homoplastomic tissue of either ΔtrnN or ΔtrnC plants could be isolated. Both mutants exhibit occasional loss of leaf sectors, and mutant plastid chromosomes are rapidly lost upon relief of selective pressure. This suggests that the knock-out of both trn genes is lethal, and that both tRNA species are required for cell survival. Surprisingly, the impact on chloroplast and cell development differs pronouncedly between the two mutants. Heteroplastomic ΔtrnC and ΔtrnN tissue exhibit different aberrations of the internal membrane systems and, more importantly, heteroplastomic ΔtrnN plants are variegated. Accumulation of tRNA-N and plastid-encoded proteins is reduced in white sectors of ΔtrnN plants, and differentiation of palisade cells is abolished. Our data demonstrate that plastid tRNAs are essential, i.e. not complemented by cytosolic tRNA, and have a differential impact on chloroplast and plant cell development.
No References
No Citations
No Supplementary Data
No Article Media
No Metrics

Keywords: chloroplast; organelle; palisade development; plastid transformation; tRNA; translation

Document Type: Research Article

Affiliations: 1: Institut für Biologie, Freie Universität Berlin, Berlin, Germany, 2: Department Biologie I, Bereich Botanik, Ludwig-Maximilians-Universität München, München, Germany, 3: ARC Centre of Excellence in Plant Energy Biology; University of Western Australia, Perth, Australia, and

Publication date: September 1, 2007

  • Access Key
  • Free content
  • Partial Free content
  • New content
  • Open access content
  • Partial Open access content
  • Subscribed content
  • Partial Subscribed content
  • Free trial content
Cookie Policy
X
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more