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Free Content EDM2 is required for RPP7-dependent disease resistance in Arabidopsis and affects RPP7 transcript levels

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Summary

Specific disease resistance of Arabidopsis thaliana against the Hyaloperonospora parasitica isolate Hiks1 (HpHiks1) is mediated by RPP7. Although this disease resistance gene encodes a typical nucleotide binding site leucine-rich repeat (NB-LRR) disease resistance protein, its function is independent of the defense hormone salicylic acid and most known genes required for plant immune responses. We identified EDM2 (enhanced downy mildew 2) in a genetic screen for RPP7 suppressors. Mutations of EDM2 phenocopy RPP7 mutations, but do not affect other tested disease resistance genes. We isolated EDM2 by map-based cloning. The predicted EDM2 protein is structurally unrelated to previously identified components of the plant immune system, bears typical features of transcriptional regulators, including plant homeodomain (PHD)-finger-like domains, and defines a plant-specific protein family. In edm2 mutants both constitutive and HpHiks1-induced RPP7 transcript levels are reduced, suggesting that EDM2 is either a direct or an indirect regulator of RPP7 expression. Microarray analyses defined a set of defense-associated genes, the expression of which is suppressed during successful HpHiks1 colonization of either rpp7 or edm2 plants. This transcriptional phenotype is counteracted by an EDM2/RPP7-dependent mechanism.
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Keywords: Arabidopsis thaliana; EDM2 (enhanced downy mildew 2)-like proteins (ELPs); Hyaloperonospora parasitica; disease resistance gene; plant homeodomain (PHD) finger

Document Type: Research Article

Affiliations: 1: Center for Plant Cell Biology, Department of Botany and Plant Sciences, University of California at Riverside, CA 92521, USA, 2: Department of Plant Pathology, Physiology, and Weed Science, and Fralin Biotechnology Center, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061-0346, USA, 3: Department of Biology, CB#3280 University of North Carolina at Chapel Hill, NC 27599, USA, 4: Warwick-HRI, University of Warwick, Warwick CV35 9EF, UK, 5: Syngenta Biotechnology, Inc., 3054 Cornwallis Road, Research Triangle Park, NC 27709, USA, and

Publication date: March 1, 2007

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