Skip to main content
padlock icon - secure page this page is secure

Free Content Transcriptionally active transposable elements in recent hybrid sugarcane

Download Article:

You have access to the full text article on a website external to Ingenta Connect.

Please click here to view this article on Wiley Online Library.

You may be required to register and activate access on Wiley Online Library before you can obtain the full text. If you have any queries please visit Wiley Online Library

Summary

Transposable elements (TEs) are considered to be important components of the maintenance and diversification of genomes. The recent increase in genome sequence data has created an opportunity to evaluate the impact of these active mobile elements on the evolution of plant genomes. Analysis of the sugarcane transcriptome identified 267 clones with significant similarity to previously described plant TEs. After full cDNA sequencing, 68 sugarcane TE clones were assigned to 11 families according to their best sequence alignment against a fully characterized element. Expression was further investigated through a combined study utilizing electronic Northerns, macroarray, transient and stable sugarcane transformation. Newly synthesized cDNA probes from flower, leaf roll, apical meristem and callus tissues confirm previous results. Callus was identified as the tissue with the highest number of TEs being expressed, revealing that tissue culture drastically induced the expression of different elements. No tissue-specific family was identified. Different representatives within a TE family displayed differential expression patterns, showing that each family presented expression in almost every tissue. Transformation experiments demonstrated that most Hopscotch clone-derived U3 regions are, indeed, active promoters, although under a strong transcriptional regulation. This is a large-scale study about the expression pattern of TEs and indicates that mobile genetic elements are transcriptionally active in the highly polyploid and complex sugarcane genome.
No References
No Citations
No Supplementary Data
No Article Media
No Metrics

Keywords: Hopscotch; Saccharum spp; expression profile; macroarrays; promoters; sugarcane; transposable elements

Document Type: Research Article

Affiliations: 1: Departamento de Botânica, IB-USP, Rua do Matão 277, São Paulo, CEP 055080-090, SP, Brazil, 2: Agilent Technologies Brasil, Al. Araguaia, 1142, Alphaville, CEP 06455-940 Barueri, SP, Brazil, 3: Centro de Biologia Molecular e Engenheria Genética, Universidade Estadual de Campinas, C.P.6010, CEP 13083-875, Campinas, SP, Brazil, and 4: Centro de Tecnologia Canavieira, Caixa Postal 162, CEP 13400-970 Piracicaba, SP, Brazil

Publication date: December 1, 2005

  • Access Key
  • Free content
  • Partial Free content
  • New content
  • Open access content
  • Partial Open access content
  • Subscribed content
  • Partial Subscribed content
  • Free trial content
Cookie Policy
X
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more