Skip to main content
padlock icon - secure page this page is secure

Free Content The mitochondrion – an organelle commonly involved in programmed cell death in Arabidopsis thaliana

Download Article:

You have access to the full text article on a website external to Ingenta Connect.

Please click here to view this article on Wiley Online Library.

You may be required to register and activate access on Wiley Online Library before you can obtain the full text. If you have any queries please visit Wiley Online Library

Summary

Plant cells undergoing programmed cell death (PCD) at late stages typically show chromatin condensation and endonucleolytic cleavage prior to obvious membrane or organelle ultrastructural changes. To investigate possible early PCD-associated events, we used microscopic observations and flow cytometry to quantitate mitochondrial membrane potential (ΔΨm) changes during PCD at the single cell and population levels using Arabidopsis protoplasts. A ΔΨm loss was commonly induced early during plant PCD and was important for PCD execution, as evidenced by the concomitant reduction of the change in ΔΨm and PCD by cyclosporin A, which inhibits mitochondrial permeability transition pores in animal cells. ΔΨm loss occurred prior to nuclear morphological changes and was only associated with mitochondrial cytochrome c release (an apoptotic trigger in animals) in response to one of three PCD elicitors. Three different stimuli in wild type implicated ΔΨm changes in PCD: ceramide, protoporphyrin IX, and the hypersensitive response elicitor AvrRpt2. Additionally, the behavior of the conditional ectopic cell death mutant accelerated cell death2 and ACD2-overproducing plants also implicated ΔΨm alteration as key for PCD execution. Because ACD2 is largely a chloroplast component in mature plants, the observation that the cell death in acd2 mutants requires changes in mitochondrial functions implicates communication between chloroplasts and mitochondria in mediating PCD activation. We suggest that ΔΨm loss is a common early marker in plant PCD, similar to what has been documented in animals. However, unlike in animal cells, in plant cells, mitochondrial cytochrome c release is not an obligatory step in PCD control.
No References
No Citations
No Supplementary Data
No Article Media
No Metrics

Keywords: ACD2; apoptosis; ceramide; flow cytometry; mitochondrial permeability transition; protoporphyrin IX

Document Type: Research Article

Affiliations: 1: Department of Molecular Genetics and Cell Biology, The University of Chicago, 1103 East 57th Street EBC410, Chicago, IL 60637, USA 2: Flow Cytometry Facility, The University of Chicago, Kovler Rm 037, Chicago, IL 60637, USA

Publication date: November 1, 2004

  • Access Key
  • Free content
  • Partial Free content
  • New content
  • Open access content
  • Partial Open access content
  • Subscribed content
  • Partial Subscribed content
  • Free trial content
Cookie Policy
X
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more