Skip to main content
padlock icon - secure page this page is secure

Free Content The plasma membrane oxidase NtrbohD is responsible for AOS production in elicited tobacco cells

Download Article:
 Download
(PDF)
 
Summary

A cDNA encoding a protein, NtrbohD, located on the plasma membrane and homologue to the flavocytochrome of the neutrophil NADPH oxidase, was cloned in tobacco. The corresponding mRNA was accumulated when tobacco leaves and cells were treated with the fungal elicitor cryptogein. After elicitation with cryptogein, tobacco cells transformed with antisense constructs of NtrbohD showed the same extracellular alkalinization as the control, but no longer produced active oxygen species (AOS). This work represents the first demonstration of the function of a homologue of gp91–phox in AOS production in elicited tobacco cells.
No References
No Citations
No Supplementary Data
No Article Media
No Metrics

Keywords: NADPH oxidase; active oxygen species; cryptogein; elicitation; plasma membrane; tobacco

Document Type: Research Article

Affiliations: Laboratoire de Phytopharmacie et Biochimie des Interactions Cellulaires, UMR692 INRA/Université de Bourgogne, BP 86510, 21065 DIJON Cedex, France

Publication date: July 1, 2002

  • Access Key
  • Free content
  • Partial Free content
  • New content
  • Open access content
  • Partial Open access content
  • Subscribed content
  • Partial Subscribed content
  • Free trial content
Cookie Policy
X
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more