Skip to main content
padlock icon - secure page this page is secure

Free Content In situ analysis of lignins in transgenic tobacco reveals a differential impact of individual transformations on the spatial patterns of lignin deposition at the cellular and subcellular levels

Download Article:

You have access to the full text article on a website external to Ingenta Connect.

Please click here to view this article on Wiley Online Library.

You may be required to register and activate access on Wiley Online Library before you can obtain the full text. If you have any queries please visit Wiley Online Library


Using tobacco transgenic lines altered in the monolignol biosynthetic pathway and which differ in their lignin profiles we have evaluated lignin deposition at the cellular and subcellular levels using several microanalytical techniques. Surprisingly, whereas a Cinnamoyl CoA reductase (CCR) down-regulated line with a strong decrease in lignin content exhibited an overall reduction in lignin deposition in the walls of the different xylem cell types, this reduction was selectively targeted to the fibers in a double transformant (down-regulated for both CCR and Cinnamyl alcohol dehydrogenase (CAD)) displaying a similar degree of global lignin content decrease. Fiber and vessel secondary walls of the transgenic tobacco line homozygous for the ccr antisense gene (CCR.H) down-regulated plants were dramatically destructured, particularly in the S2 sublayer, whereas the deposition of lignins in the S1 sublayer was not significantly modified. In contrast, cell wall organization was slightly altered in xylem cells of the double transformant. The relative distribution of non-condensed and condensed units in lignin, evaluated microscopically with specific antibodies, was differentially affected in the transgenics studied and, in a general way, a drop in non-condensed lignin units (β− 0–4 interunit linkages) was associated with a loss of cohesion and extensive disorganization of the secondary wall. These results demonstrate that lignification is tightly and independently regulated in individual cell types and cell wall sublayers. They also show that down-regulation of specific genes may induce targeted changes in lignin structure and in spatial deposition patterns of the polymer.
No References
No Citations
No Supplementary Data
No Article Media
No Metrics

Keywords: lignification; lignin deposition; transgenic tobacco; vessels and fibers; wall layers

Document Type: Research Article

Affiliations: 1: Laboratoire de Biochimie, INRA centre de Reims, 2 Esplanade R. Garros, BP 224, 51686 Reims cedex 2, France, and 2: Institut Fédératif de Recherche Fr40 ‘Signalisation Cellulaire et Biotechnologie végétale’, Pôle de Biotechnologie végétale, BP 27, 31326 Castanet Tolosan cedex, France 3: Centre de Recherches sur les Macromolécules Végétales, CNRS, BP 53, 38041 Grenoble Cedex 09, France, 4: UMR CNRS/UPS 5546, Signaux et Messages Cellulaires chez les Végétaux, Pôle de Biotechnologie Végétale, 24 Chemin de Borde-Rouge – BP 17 Auzeville – 31326 Castanet-Tolosan, France,

Publication date: November 1, 2001

  • Access Key
  • Free content
  • Partial Free content
  • New content
  • Open access content
  • Partial Open access content
  • Subscribed content
  • Partial Subscribed content
  • Free trial content
Cookie Policy
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more