Skip to main content
padlock icon - secure page this page is secure

Free Content A method for isolating a high yield of Arabidopsis chloroplasts capable of efficient import of precursor proteins

Download Article:
 Download
(PDF)
 
Summary

Chloroplasts were isolated from Arabidopsis plants grown under different conditions, and using different protocols, to determine a method that would yield chloroplasts capable of binding and importing precursor proteins. Chloroplasts isolated from protoplasts and purified on a Percoll gradient were highly import-competent, with little non-specific binding of the precursor, and a high yield of intact chloroplasts (0.1 mg chlorophyll/g FW). Chloroplasts from plants grown on agar plates had a much higher rate of import than those from plants grown on soil. Protein import remained high at all of the ages tested for chloroplasts from plate-grown plants, whereas it declined during the development of soil-grown plants. Arabidopsis chloroplasts imported a range of precursor proteins and had nucleotide requirements for binding and import similar to those reported for pea chloroplasts.
No References
No Citations
No Supplementary Data
No Article Media
No Metrics

Keywords: ATP; Arabidopsis; chloroplast isolation; protein import

Document Type: Research Article

Affiliations: MSU-Department of Energy Plant Research Laboratory, Michigan State University, East Lansing, MI 48824-1312, USA

Publication date: July 1, 2001

  • Access Key
  • Free content
  • Partial Free content
  • New content
  • Open access content
  • Partial Open access content
  • Subscribed content
  • Partial Subscribed content
  • Free trial content
Cookie Policy
X
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more