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Free Content Differential regulation of three functional ammonium transporter genes by nitrogen in root hairs and by light in leaves of tomato

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Summary

To elucidate the role of NH 4  +  transporters in N nutrition of tomato, two new NH 4  +  transporter genes were isolated from cDNA libraries of root hairs or leaves of tomato. While LeAMT1;2 is closely related to LeAMT1;1 (75.6% amino acid identity), LeAMT1;3 is more distantly related (62.8% identity) and possesses two short upstream open reading frames in the 5′ end of the mRNA and a particularly short N-terminus of the protein as unique features. When expressed in yeast mutants defective in NH 4  +  uptake, all three genes complemented NH 4  +  uptake. In roots of hydroponically grown plants, transcript levels of LeAMT1;2 increased after NH 4  +  or NO3 supply, while LeAMT1;1 was induced by N deficiency coinciding with low glutamine concentrations, and LeAMT1;3 was not detected. In aeroponic culture, expression of LeAMT1;1 and LeAMT1;2 was higher in root hairs than in the remaining root fraction. Growth of plants at elevated CO2 slightly decreased expression of LeAMT1;2 and LeAMT1;3 in leaves, but strongly repressed transcript levels of chloroplast glutamine synthetase and photorespiratory serine hydroxymethyl-transferase. Expression of LeAMT1;2 and LeAMT1;3 showed a reciprocal diurnal regulation with highest transcript levels of LeAMT1;3 in darkness and highest levels of LeAMT1;2 after onset of light. These results indicate that in tomato at least two high-affinity NH 4  +  transporters, LeAMT1;1 and LeAMT1;2, are differentially regulated by N and contribute to root hair-mediated NH 4  +  acquisition from the rhizosphere. In leaves, the reciprocally expressed transporters LeAMT1;2 and LeAMT1;3 are supposed to play different roles in N metabolism, NH 4  +  uptake and/or NH3 retrieval during photorespiration.
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Document Type: Research Article

Affiliations: 1: Pflanzenphysiologie, Zentrum für Molekularbiologie der Pflanzen Tübingen (ZMBP), Morgenstelle 1, 72076 Tübingen, Germany, and 2: Institut für Pflanzenernährung, Universität Hohenheim, 70593 Stuttgart, Germany

Publication date: January 1, 2000

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