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Free Content Emission of nitrous oxide (N2O) from transgenic tobacco expressing antisense NiR mRNA

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The emission of N2 and N2O from intact transgenic tobacco (clone 271) expressing antisense nitrite reductase (NiR) mRNA, and wild-type plants grown aseptically, on NO3, NO2 or NH4+ -containing medium was investigated. 15N contents of gas sampled from gas-sealed pots, in which the plants were grown on 15N-containing medium, were analyzed by gas chromato- graphy and mass spectrometry (GC–MS). No emission of N2 was detected in either of the gas samples from plant clone 271 or the wild-type grown on NO3-containing medium. N2O emission from clone 271 grown on NO3-containing medium was detected, but not from the wild-type plants. The N2O emission rate of clone 271 was 106 ng N2O mg–1 incorporated N week–1 and the N2O emission was inhibited by tungstate (a nitrate reductase inhibitor). No emission of N2O was found from clone 271 or wild-type plants grown on medium containing NH4+. Emission of N2O also was detected from clone 271 grown on NO2-containing medium and its emission rate increased with increasing NO2 levels in plants. We speculate that NO3 is reduced to NO2 and that a part of NO2 is metabolized to N2O in clone 271.
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Document Type: Short Communication

Affiliations: 1: Department of Mathematical and Life Science, Graduate School of Science, Hiroshima University, Kagamiyama 1-3-1, Higashi-Hiroshima, 739-8526 Japan, and 2: Laboratoire de Biologie Cellulaire, INRA, Center de Versailles, Route de St Cyr, 78026 Versailles Cedex, France

Publication date: July 1, 1999

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