The Pib gene for rice blast resistance belongs to the nucleotide binding and leucine-rich repeat class of plant disease resistance genes
Rice blast, caused by the fungal pathogen Magnaporthe grisea, is one of the most serious diseases of rice. Here we describe the isolation and characterization of Pib, one of the rice blast resistance genes. The Pib gene was isolated by a map-based cloning strategy. The deduced amino acid sequence of the Pib gene product contains a nucleotide binding site (NBS) and leucine-rich repeats (LRRs); thus, Pib is a member of the NBS-LRR class of plant disease resistance genes. Interestingly, a duplication of the kinase 1a, 2 and 3a motifs of the NBS region was found in the N-terminal half of the Pib protein. In addition, eight cysteine residues are clustered in the middle of the LRRs, a feature which has not been reported for other R genes. Pib gene expression was induced upon altered environmental conditions, such as altered temperatures and darkness.
Document Type: Original Article
Affiliations: 1: Rice Genome Research Program, Institute of the Society for Techno-Innovation of Agriculture, Forestry and Fisheries, Ippaizuka 446–1, Kamiyokoba, Tsukuba, Ibaraki 305–0854, Japan, 2: Rice Genome Research Program, National Institute of Agrobiological Resources, Kannondai 2-1-2, Tsukuba, Ibaraki 305–8602, Japan, 3: Miyagi Prefecture Furukawa Agricultural Experiment Station, Suwa 1-4-30, Furukawa, Miyagi 989–6175, Japan, and
Publication date: July 1, 1999