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Free Content Regulation of a putative high-affinity nitrate transporter (Nrt2;1At) in roots ofArabidopsis thaliana

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Putative high-affinity nitrate (NO3) transporter genes, designatedNrt2;1AtandNrt2;2At, were isolated fromArabidopsis thalianaby RT–PCR using degenerate primers. The genes shared 86% and 89% identity at the amino acid and nucleotide levels, respectively, while their proteins shared 30–73% identities with other eukaryotic highaffinity NO3 transporters. Both genes were induced by NO3, butNrt2;1Atgene expression was not apparent in 2- and 5-day-old plants. By 10 days, and thereafter,Nrt2;1Atgene expression in roots was substantially higher than for theNrt2;2Atgene. RootNrt2;1Atexpression levels were strongly correlated with inducible high-affinity 13NO3 influx into intact roots under several treatment conditions. The use of inhibitors of N assimilation indicated that downregulation ofNrt2;1Atexpression was mediated by NH4+, gln and other amino acids.
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Document Type: Short Communication

Affiliations: Botany Department, University of British Columbia, Vancouver, BC, Canada V6T 1Z4

Publication date: March 1, 1999

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