Skip to main content
padlock icon - secure page this page is secure

Free Content Changes in aldolase activity in wild-type potato plants are important for acclimation to growth irradiance and carbon dioxide concentration, because plastid aldolase exerts control over the ambient rate of photosynthesis across a range of growth conditions

Download Article:

You have access to the full text article on a website external to Ingenta Connect.

Please click here to view this article on Wiley Online Library.

You may be required to register and activate access on Wiley Online Library before you can obtain the full text. If you have any queries please visit Wiley Online Library

Summary

Even though plastid aldolase catalyses a reversible reaction, does not possess properties allowing it to contribute to ‘fine’ regulation, and would therefore be considered unimportant for the control of metabolism and growth, antisense transformants with a 50–70% decrease in aldolase activity showed an inhibition of photosynthesis and growth. We now show that acclimation of photosynthesis to growth conditions includes and requires changes in plastid aldolase activity. Wild-type potato plants and transformants were grown at low irradiance (70 mol m–2 sec–1), and at high irradiance (390 mol m–2 sec–1) at 400 or 800 p.p.m. carbon dioxide. (i) Ambient photosynthesis was always inhibited by a 30–40% decrease of aldolase activity, the strongest inhibition being observed when plants were growing in high irradiance and elevated carbon dioxide. (ii) The inhibition was due to a low rate of ribulose-1,5-bisphosphate regeneration in low light, exacerbated by an inadequate rate of starch synthesis in high light and elevated carbon dioxide. Decreased expression of aldolase in antisense transformants was also accompanied by a decrease of fructose-1,6-bisphosphatase protein and activity, and Rubisco activity. Transcript levels for the plastid fructose-1,6-bisphosphatase and the small subunit of Rubisco did not decrease. (iii) In wild-type plants, increasing the growth irradiance from 70 to 390 mol m–2 sec–1 led to a 15–95% increase of the activity of eight Calvin cycle enzymes, and increasing the carbon dioxide concentration from 400 to 800 p.p.m. led to a 5–35% decrease of these enzyme activities. The largest changes occurred for aldolase, and for transketolase which also catalyses a reversible reaction and is not subject to ‘fine’ regulation.
No References
No Citations
No Supplementary Data
No Article Media
No Metrics

Document Type: Original Article

Affiliations: 1: Botanisches Institut, Im Neuenheimer Feld 360, Universität Heidelberg, D-69120 Heidelberg, Germany, and 2: Institut für Pflanzenernährung (330), Universität Hohenheim, 70593 Stuttgart, Germany

Publication date: March 1, 1999

  • Access Key
  • Free content
  • Partial Free content
  • New content
  • Open access content
  • Partial Open access content
  • Subscribed content
  • Partial Subscribed content
  • Free trial content
Cookie Policy
X
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more