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Free Content Regulation of gibberellin biosynthesis genes during flower and early fruit development of tomato

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Summary

Gibberellins (GAs) are essential for the development of fertile flowers in tomato, and may also be required immediately after fertilization. In the GA-biosynthetic pathway, the reactions catalyzed by GA 20-oxidases have been implicated as site of regulation. To study the regulation of GA biosynthesis in flower and early fruit development, we isolated three tomato GA 20-oxidase cDNA clones,Le20ox-1, -2 and -3. The three genes showed different organ-specific patterns of mRNA accumulation. Analysis of the transcript levels of the threeGA 20-oxidasegenes, as well as those ofcopalyl diphosphate synthase(LeCPS) andGA 3-hydroxylase (Le3OH-2)during flower bud and early fruit development, revealed temporally distinct patterns of mRNA accumulation. Up until anthesis, transcripts were observed forLeCPS, Le20ox-1, -2 and Le3OH-2, with an accumulation ofLe20ox-1mRNA. In contrast to the high level ofLe3OH-2transcripts in the fully open flower, mRNA levels ofLe20ox-1, -2 and LeCPSwere reduced at this stage. After anthesis,LeCPSandLe20ox-1transcripts increased again. In addition,Le20ox-3transcripts increased whereas the transcripts ofLe3OH-2decreased to an undetectable level.In situhybridization results demonstrated that during early stages of bud development,Le20ox-2transcripts were localized in the tapetum and placenta. The presented results supply novel data about localization of GA biosynthesis gene transcripts, and indicate that transcript levels of GA biosynthesis genes are all highly regulated during flower bud development.
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Document Type: Original Article

Affiliations: Frontier Research Program, The Institute of Physical and Chemical Research (RIKEN), Wako-shi, Saitama 351–0198, Japan

Publication date: February 1, 1999

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