Skip to main content
padlock icon - secure page this page is secure

Free Content A single base change prevents import of cytosolic tRNAAla into mitochondria in transgenic plants

Download Article:

You have access to the full text article on a website external to Ingenta Connect.

Please click here to view this article on Wiley Online Library.

You may be required to register and activate access on Wiley Online Library before you can obtain the full text. If you have any queries please visit Wiley Online Library

Plant mitochondria do not contain a full set of tRNA genes, and the additional tRNAs needed for protein synthesis (including tRNAAla) are imported from the cytosol. The import process appears to be highly specific for certain tRNAs, and it has been suggested that the cognate aminoacyl-tRNA synthetases may be responsible for this specificity. In order to test this, we have grown transgenic tobacco plants expressing Arabidopsis thaliana tRNAAla carrying a U70 to C70 mutation, which we have previously shown blocks aminoacylation by the plant alanyl-tRNA synthetase. Unlike the wild-type tRNAAla, the mutant tRNA is not present in the mitochondrial tRNA fraction. This is the first report of a tRNA mutation which prevents mitochondrial import and strongly supports the hypothesis that aminoacyl-tRNA synthetases are involved in this process in plants. Insertion of four bases into the anticodon loop of tRNAAla does not prevent mitochondrial import, implying that the tRNA might not need to participate in translation to be imported.
No References
No Citations
No Supplementary Data
No Article Media
No Metrics

Document Type: Short Communication

Affiliations: 1: Institut de Biologie Moléculaire des Plantes du CNRS, Université Louis Pasteur, 12 rue du Général Zimmer, F-67084 Strasbourg-Cedex, France 2: Station de Génétique et d’Amélioration des Plantes, INRA, Route de St-Cyr, 78026 Versailles-Cedex, France

Publication date: November 1, 1996

  • Access Key
  • Free content
  • Partial Free content
  • New content
  • Open access content
  • Partial Open access content
  • Subscribed content
  • Partial Subscribed content
  • Free trial content
Cookie Policy
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more