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Free Content PCR diagnosis of visceral leishmaniasis in an endemic region, Mymensingh district, Bangladesh

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Summary Detection of parasites in a clinical sample is necessary to confirm a suspected case of leishmaniasis. We compared the sensitivity of internal transcribed spacer 1-PCR (ITS 1-PCR) assay for parasite diagnosis with that of microscopic detection in clinical samples from kala-azar (KA) or post-kala-azar dermal leishmaniasis (PKDL) suspects in Mymensingh. Of 39 specimens collected from 35 KA and four PKDL suspects, 26 were positive by microscopic examination of smears from bone marrow and skin exudates; 38 specimens spotted on filter paper and 27 of the 28 Giemsa-stained slides tested by PCR proved positive by ITS1-PCR.

La détection du parasite Leishmania dans un échantillon clinique est nécessaire pour confirmer un cas suspect de leishmaniose. Nous avons comparé la sensibilité du test ‘Internal Spacer Transcrit 1-PCR’ (ITS1-PCR) pour le diagnostic parasitologique à celui du diagnostic par détection microscopique dans des échantillons cliniques de cas suspects de leishmaniose cutanée kala-azar (KA) ou post-kala-azar (PKDL) à Mymensingh. Sur 39 échantillons collectés chez 35 suspects KA et 4 PKDL, 26 étaient positifs par examen microscopique des frottis de la moelle osseuse et les exsudats de la peau, 38 spécimens collectés sur papier-filtre et 27 des 28 lames colorées au Giemsa se sont révélés positifs par le test ITS1-PCR.
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Keywords: Bangladesh; ITS1-PCR; Kala-azar; Microscopía; epidemiology; epidemiología; internal transcribed spacer 1-polymerase chain reaction; kala-azar; microscopie; microscopy; épidémiologie

Document Type: Research Article

Affiliations: 1:  Department of Microbiology, Mymensingh Medical College, Mymensingh, Bangladesh 2:  Institut für Mikrobiologie und Hygiene, Charité Universitätsmedizin Berlin, Berlin, Germany

Publication date: May 1, 2009

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