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Genetic diversity associated with in vitro and conventional bud propagation of Saccharum varieties using RAPD analysis

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Polymorphisms in the genomic DNA of eight varieties maintained by conventional bud propagation (via rhizomes) and by in vitro shoot tip cultures were detected by RAPD analysis of sugarcane varieties. The study estimated the genetic diversity induced after in vitro multiplication of these varieties. Higher (28.9%) and lower (12%) numbers of polymorphic bands were detected in plants propagated via rhizomes; the genetic similarity estimate varying from 0.63 to 0.80. Plants of SP90-3723 and SP91-1049, or RB85-5113 and SP90-3723, varieties involving greater genetic distances may be indicated as progenitors in breeding programmes. In vitro multiplication of RB86-7515, RB85-5113, RB83-5054 and SP86-42 varieties increases genetic variability, while in vitro multiplication of SP91-1049, SP90-1638, RB92-8064 and SP90-3723 leads to genetic similarity. Results show that the RAPD technique is an effective tool for detecting polymorphism in sugarcane clones and it allows quick collection of the necessary information (more genetically divergent plant varieties) to guide new crossing in sugarcane breeding programmes.
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Keywords: RAPD analysis; Saccharum cultivars; genetic diversity; in vitro culture

Document Type: Research Article

Affiliations: Department of Cell Biology and Genetics, State University of Maringá, 87020-900 Maringá, Brazil

Publication date: 01 April 2008

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