Skip to main content
padlock icon - secure page this page is secure

Molecular, phylogenetic and comparative genomic analysis of the cytokinin oxidase/dehydrogenase gene family in the Poaceae

Buy Article:

$43.00 + tax (Refund Policy)

Summary

The genomes of cereals such as wheat (Triticum aestivum) and barley (Hordeum vulgare) are large and therefore problematic for the map‐based cloning of agronomicaly important traits. However, comparative approaches within the Poaceae permit transfer of molecular knowledge between species, despite their divergence from a common ancestor sixty million years ago. The finding that null variants of the rice gene cytokinin oxidase/dehydrogenase 2 (OsCKX2) result in large yield increases provides an opportunity to explore whether similar gains could be achieved in other Poaceae members. Here, phylogenetic, molecular and comparative analyses of CKX families in the sequenced grass species rice, brachypodium, sorghum, maize and foxtail millet, as well as members identified from the transcriptomes/genomes of wheat and barley, are presented. Phylogenetic analyses define four Poaceae CKX clades. Comparative analyses showed that CKX phylogenetic groupings can largely be explained by a combination of local gene duplication, and the whole‐genome duplication event that predates their speciation. Full‐length OsCKX2 homologues in barley (HvCKX2.1, HvCKX2.2) and wheat (TaCKX2.3, TaCKX2.4, TaCKX2.5) are characterized, with comparative analysis at the DNA, protein and genetic/physical map levels suggesting that true CKX2 orthologs have been identified. Furthermore, our analysis shows CKX2 genes in barley and wheat have undergone a Triticeae‐specific gene‐duplication event. Finally, by identifying ten of the eleven CKX genes predicted to be present in barley by comparative analyses, we show that next‐generation sequencing approaches can efficiently determine the gene space of large‐genome crops. Together, this work provides the foundation for future functional investigation of CKX family members within the Poaceae.
No References
No Citations
No Supplementary Data
No Article Media
No Metrics

Document Type: Research Article

Affiliations: 1: National Institute of Agricultural Botany (NIAB), Cambridge, UK 2: Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), Gatersleben, Germany 3: Leibniz Institute of Age Research—Fritz-Lipmann-Institute (FLI), Jena, Germany 4: RAGT Seeds Ltd., Ickelton, UK 5: KWS UK Ltd., Thriplow, Royston, UK 6: Department of Plant Sciences, University of Cambridge, Cambridge, UK

Publication date: 01 January 2012

  • Access Key
  • Free content
  • Partial Free content
  • New content
  • Open access content
  • Partial Open access content
  • Subscribed content
  • Partial Subscribed content
  • Free trial content
Cookie Policy
X
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more